Reactive Oxygen Species (ROS) Fluorometric Assay Kit

• Target:

  ROS; DCFH-DA; DCFH; DCF

• Type:

  Fluorometric method

• Field of Research:

  Oxidative Stress; Ferroptosis; Metabolic Diseases

• Assay Principle:

  DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin) . In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

• Assay Performance Time:

  60 min

• Sample Type:

  Fresh tissue; cultured cells

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S2211383522004798

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S0043135420312884

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S0891584922009455

• Citation 04:

  https://www.mdpi.com/1983336

• Citation 05:

  https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.14085

• Citation 06:

  https://journals.asm.org/doi/abs/10.1128/Spectrum.00074-21

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S0147651322009770

• Citation 08:

  https://www.ahajournals.org/doi/abs/10.1161/hypertensionaha.118.11035

• Citation 09:

  https://www.tandfonline.com/doi/abs/10.1080/10717544.2022.2147280

• Citation 10:

  https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/biof.1824

• Citation 11:

  https://journals.sagepub.com/doi/abs/10.1177/0271678X211029060

• Citation 12:

  https://static.frontiersin.org/articles/10.3389/fmolb.2022.816510/full

• Detection Instrument:

  Fluorescence Microplate reader; Fluorescence Microscope; Flow Cytometry