Vitamin C (VC) Colorimetric Assay Kit

• Target:

  VC; Ascorbic acid

• Type:

  Colorimetric method

• Field of Research:

  Oxidative stress; Plant Physiology Series

• Assay Principle:

  The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.

• Assay Performance Time:

  80 min

• Sample Type:

  Serum; plasma; tissue

• Detection Range:

  0.35-20 μg/mL

• Sensitivity:

  0.35 μg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://www.mdpi.com/article/10.3390/nu15040832

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S0891584920310911

• Citation 03:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/

• Citation 04:

  https://www.nature.com/articles/s41598-019-52646-z

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S0024320520303325

• Citation 06:

  https://www.tandfonline.com/doi/abs/10.2147/IJGM.S402206

• Citation 07:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/jez.2603

• Inter CV:

  3.3%

• Intra CV:

  2.3%

• Detection Instrument:

  Spectrophotometer