Aspartate Aminotransferase (AST/GOT) Activity Fluorometric Assay Kit

• Target:

  AST; GOT

• Type:

  Fluorometric method

• Field of Research:

  Liver and Renal Function

• Assay Principle:

  AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.

• Assay Performance Time:

  80 min

• Sample Type:

  Cell; animal tissue; serum (plasma) ; other liquid samples

• Detection Range:

  0.03-1.5 U/L

• Sensitivity:

  0.03 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  7.3%

• Intra CV:

  2.2%

• Detection Instrument:

  Fluorescence microplate reader