Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

• Target:

  ALT

• Type:

  Fluorometric method

• Field of Research:

  Liver and Renal Function

• Assay Principle:

  ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

• Assay Performance Time:

  75 min

• Sample Type:

  Serum; plasma; animal tissue

• Detection Range:

  0.01-0.83 U/L

• Sensitivity:

  0.01 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S1742706120307509

• Citation 02:

  https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332

• Citation 03:

  https://pubs.acs.org/doi/abs/10.1021/acsami.9b19518

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S0278691521001575

• Citation 05:

  https://www.mdpi.com/2039948

• Citation 06:

  https://www.nature.com/articles/s41598-022-21816-x

• Inter CV:

  9.8%

• Intra CV:

  2.3%

• Detection Instrument:

  Fluorescence Microplate Reader