Biotin Quantitative Determination ELISA Kit

Biotin Quantitative Determination ELISA Kit

• CAS Number: 7732-18-5
• Type: Competitive-ELISA
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate providedin this kit has been pre-coated with Biotin. During the reaction, Biotin in samples orStandard competes with a fixed amount of Biotin on the solid phase supporter for sites onthe Avidin-HRP specific to Biotin. Excess Avidin-HRP and unbound sample or standardare washed from the plate. Then a TMB substrate solution is added to each well. Theenzyme-substrate reaction is terminated by the addition of stop solution and the colorchange is measured spectrophotometrically at a wavelength of 450±2 nm. Theconcentration of Biotin in the samples is then determined by comparing the OD of thesamples to the standard curve.
• Assay Performance Time: 1 h 30 min
• Detection Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 12 months