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Mouse MBP (Myelin Basic Protein) CLIA Kit

CAT: 0763-E-CL-M0504-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-CL-M0504-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
MBP
UniProt
P04370
Cross Reactivity
This kit recognizes Mouse MBP in samples.No significant cross-reactivity or interference between Mouse MBP and analogues was observed
Target
Myelin_MBP
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Neuroscience; Tags and Cell Markers
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MBP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse MBP. You can calculate the concentration of Mouse MBP in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse MBP concentrations in Serum, plasma and other biological fluids.

UniProtKB · P04370

Myelin basic protein

MBP_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P04370
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Mbp
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
MBP
EC number
—
Processing
—
Secondary accessions
Q01585, Q03139, Q03176, Q61836, Q61837, Q99KE4, Q9QWP1
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationCitrullinationMethylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Disease

Autoimmune encephalomyelitis

Cellular component

Cell membraneCytoplasmMembraneNucleus

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