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Mouse GAL3 (Galectin 3) CLIA Kit

CAT: 0763-E-CL-M0319-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-CL-M0319-01Size:24 Tests
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Abbreviation
GAL3
UniProt
P16110
Cross Reactivity
This kit recognizes Mouse GAL3 in samples.No significant cross-reactivity or interference between Mouse GAL3 and analogues was observed
Target
LGALS3; CBP35; GALBP; GALIG; L31; LGALS2; MAC2; Lectin; Galactoside-Binding Soluble 3
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Cell Biology; Neuroscience
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse GAL3. You can calculate the concentration of Mouse GAL3 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
12.5-800 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
7.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse GAL3 concentrations in serum, plasma and other biological fluids.

UniProtKB · P16110

Galectin-3

LEG3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P16110
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Lgals3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
Gal-3
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationDisulfide bondPhosphoprotein

Cellular component

CytoplasmNucleusSecretedSpliceosome

Biological process

DifferentiationImmunityInnate immunitymRNA processingmRNA splicing

Molecular function

IgE-binding protein

Ligand

Lectin

Domain

Repeat

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