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Mouse PRL (Prolactin) CLIA Kit

CAT: 0763-E-CL-M0070-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-CL-M0070-01Size:24 Tests
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Abbreviation
PRL
UniProt
P06879
Cross Reactivity
This kit recognizes Mouse PRL in samples.No significant cross-reactivity or interference between Mouse PRL and analogues was observed
Target
Lactotrope; LTH; Luteotropic Hormone
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Signal transduction
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PRL. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse PRL. You can calculate the concentration of Mouse PRL in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse PRL concentrations in serum, plasma and other biological fluids.

UniProtKB · P06879

Prolactin

PRL_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06879
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Prl
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
PRL
EC number
—
Processing
Precursor
Secondary accessions
Q544X8
Protein keywords

PTM

Disulfide bondPhosphoprotein

Molecular function

Hormone

Biological process

Lactation

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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