Mouse LEP (Leptin) CLIA Kit
CAT:
763-E-CL-M0037-02
Size:
48 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Mouse LEP (Leptin) CLIA Kit
- Abbreviation: LEP
- Synonyms: LEPD;OB;OBS;Obesity Homolog;
- UniProt: P4116N/A
- Species Reactivity: Mouse
- Type: Sandwich-ELISA
- Applications: CLIA
- Field of Research: Cancer;Cardiovascular;Metabolism;Neuroscience;Signal transduction;Stem cells
- Detection Method: Chemiluminescence
- Assay Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the RLU value of the samples to the standard curve.
- Assay Performance Time: 3 h 30 min
- Detection Range: 15.63-1000 pg/mL
- Sensitivity: 9.38 pg/mL
- Shipping Conditions: Ice bag
- Storage Conditions: 2-8°C/-20°C, 6 months