Human DAT (Dopamine Transporter) CLIA Kit

CAT: 0763-E-CL-H0799-01Size: 24 TestsDry Ice: NoHazardous: No

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CAT#:0763-E-CL-H0799-01Size:24 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Abbreviation

DAT

UniProt

Q01959

Cross Reactivity

This kit recognizes Human DAT in samples.No significant cross-reactivity or interference between Human DAT and analogues was observed

Type

Sandwich-ELISA

Applications

CLIA

Field of Research

Cancer; Neuroscience

Assay Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DAT. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAT, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human DAT. You can calculate the concentration of Human DAT in the samples by comparing the RLU value of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,6 months

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human DAT concentrations in Serum, plasma and other biological fluids.

UniProtKB · Q01959

Sodium-dependent dopamine transporter

SC6A3_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q01959
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: SLC6A3
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: DA transporter, DAT
EC number: —
Processing: —
Secondary accessions: A2RUN4, Q14996

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cell membraneCell projectionMembrane

Disease

Disease variantDystoniaNeurodegenerationParkinsonism

PTM

Disulfide bondGlycoprotein

Ligand

Metal-bindingSodium

Biological process

Neurotransmitter transportSymportTransport

Domain

TransmembraneTransmembrane helix

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Alternative Products

Catalog	Name
MBS8425332-01	Human Dopamine Transporter CLIA Kit
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MBS2530843-01	Rat DAT (Dopamine Transporter) CLIA Kit
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