QuicKey Pro Human CRP (C-Reactive Protein) ELISA Kit

QuicKey Pro Human CRP (C-Reactive Protein) ELISA Kit

• Abbreviation: CRP
• Synonyms: C-Reactive Protein;CRP;PTX1;
• CAS Number: 7732-18-5
• UniProt: PN/A2741
• Species Reactivity: Human
• Type: Sandwich-ELISA
• Applications: ELISA
• Field of Research: Cancer;Cardiovascular;Epigenetics and Nuclear Signaling;Metabolism;Immunology
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CRP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human CRP. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 1 h 30 min
• Detection Range: 125-8000 pg/mL
• Sensitivity: 52.1 pg/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 6 months