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Mini Sample Mouse NGF (Nerve growth factor) ELISA Kit

CAT: 0763-E-MSEL-M0056-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-MSEL-M0056-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
NGF
UniProt
P01139
Cross Reactivity
This kit recognizes Rat PAI1 in samples. No significant cross-reactivity or interference between Rat PAI1 and analogues was observed.
Target
Beta-NGF; HSAN5; NGFB; NGF-B
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Neuroscience
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NGF. You can calculate the concentration of Mouse NGF in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.65 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse NGF concentrations in serum, plasma and other biological fluids.

UniProtKB · P01139

Beta-nerve growth factor

NGF_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01139
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ngf
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
Beta-NGF
EC number
—
Processing
Precursor
Secondary accessions
Q63864, Q6LDB7
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Cellular component

EndosomeSecreted

Molecular function

Growth factorMetalloenzyme inhibitorMetalloprotease inhibitorProtease inhibitor

Ligand

Lipid-binding

Domain

Signal

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