Mini Sample Mouse IgM (Immunoglobulin M) ELISA Kit

CAT: 0763-E-MSEL-M0044-01Size: 24 TestsDry Ice: NoHazardous: No

1 / 1

CAT#:0763-E-MSEL-M0044-01Size:24 Tests

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Abbreviation

IgM

UniProt

P01872

Cross Reactivity

This kit recognizes Mouse IgM in samples. No significant cross-reactivity or interference between Mouse IgM and analogues was observed.

Target

IgM

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgM. You can calculate the concentration of Mouse IgM in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.21 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IgM concentrations in serum, plasma and other biological fluids.

UniProtKB · P01872

Immunoglobulin heavy constant mu

IGHM_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: P01872
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Ighm
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: —
EC number: —
Processing: —
Secondary accessions: P01873

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoprotein

Domain

Immunoglobulin domainTransmembraneTransmembrane helix

Related Products

Catalog	Name
E-MSEL-M0079-01	Mini Sample Mouse IgA (Immunoglobulin A) ELISA Kit
E-MSEL-M0089-01	Mini Sample Mouse IgE (Immunoglobulin E) ELISA Kit
E-MSEL-M0066-01	Mini Sample Mouse IgG (Immunoglobulin G) ELISA Kit
E-MSEL-H0008-01	Mini Sample Human IgE (Immunoglobulin E) ELISA Kit
E-EL-M0695	ELISA kit for Mouse IgM (Immunoglobulin M)
E-EL-M3036-01	Mouse IgM (Immunoglobulin M) ELISA Kit
E-EL-P2269-01	Porcine IgM-Immunoglobulin M- ELISA Kit
E-EL-H1814-01	Human IgM (Immunoglobulin M) ELISA Kit
E-EL-R0519	ELISA kit for Rat IgM (Immunoglobulin M)
E-EL-R3054-01	Rat IgM (Immunoglobulin M) ELISA Kit

Alternative Products

Catalog	Name
abx254203-01	Low Sample Volume Mouse Immunoglobulin A (IgA) ELISA Kit
MBS2578093-01	Mini Sample IgM (Immunoglobulin M) ELISA Kit
201-16-0143	Chicken Immunoglobulin G2a (IgG2a) ELISA Kit-sample
MBS2578121-01	Mini Sample IgG (Immunoglobulin G) ELISA Kit
TRV-054774-01	Low Sample Volume ELISA Kit for Immunoglobulin A (IgA)
201-16-0142	Chicken Immunoglobulin G1 (IgG1) ELISA Kit-sample
abx350127-01	Low Sample Volume Mouse Immunoglobulin M (IgM) ELISA Kit
abx350125-01	Low Sample Volume Mouse Immunoglobulin G (IgG) ELISA Kit
abx573497-01	Low Sample Volume Cow Immunoglobulin A (IgA) ELISA Kit
TRV-054805-01	Low Sample Volume ELISA Kit for Immunoglobulin G1 (IgG1)