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Mini Sample Mouse REN (Renin) ELISA Kit

CAT: 0763-E-MSEL-M0035-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-MSEL-M0035-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
REN
UniProt
P06281
Cross Reactivity
This kit recognizes Mouse REN in samples.No significant cross-reactivity or interference between Mouse REN and analogues was observed
Target
REN
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Metabolism; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse REN. You can calculate the concentration of Mouse REN in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.52 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.ahajournals.org/doi/abs/10.1161/HYPERTENSIONAHA.119.12941
Citation 02
https://www.frontiersin.org/articles/10.3389/fcvm.2020.00135/full?utm_source=researcher_app&utm_medium=referral&utm_campaign=RESR_MRKT_Researcher_inbound
Citation 03
https://www.sciencedirect.com/science/article/pii/S0271531720304450
Citation 04
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007412/
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse REN concentrations in serum, plasma and other biological fluids.

UniProtKB · P06281

Renin-1

RENI1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06281
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ren1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.23.15
Processing
Precursor
Secondary accessions
P97911, Q543E5, Q62153, Q62154
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

Aspartyl proteaseHydrolaseProtease

PTM

Disulfide bondGlycoproteinZymogen

Cellular component

MembraneSecreted

Domain

Signal

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