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Mini Sample Mouse SELE (E-selectin) ELISA Kit

CAT: 0763-E-MSEL-M0014-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#: E-MSEL-M0014-01Size: 24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
SELE
UniProt
Q00690
Cross Reactivity
This kit recognizes Mouse SELE in samples.No significant cross-reactivity or interference between Mouse SELE and analogues was observed
Target
SELE
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Immunology; Stem cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SELE. You can calculate the concentration of Mouse SELE in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/
Citation 02
https://www.mdpi.com/2072-6694/12/3/623
Citation 03
https://www.sciencedirect.com/science/article/pii/S0891584919308792
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse SELE concentrations in serum, plasma and other biological fluids.

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