Rabbit APOE (Apolipoprotein E) ELISA Kit

Rabbit APOE (Apolipoprotein E) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit APOE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit APOE in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
• UniProt: P18287
• Field of Research: Metabolic pathway; Cardiovascular biology; Hepatology.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 50 µg/mL
• Sample Type: Serum, plasma and other biological fluids.
• Detection Range: 0.78-50 µg/mL
• Sensitivity: 0.25 µg/mL