Rat VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit

CAT:

763-E-EL-R2603-03

Size:

24 Tests

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Rat VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit

Abbreviation: VEGF-A
CAS Number: 7732-18-5
UniProt: P16612
Species Reactivity: Rat
Type: Sandwich-ELISA
Applications: ELISA
Field of Research: Cancer;Cardiovascular;Metabolism;Developmental biology;Signal transduction
Detection Method: Colormetric
Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VEGF-A. You can calculate the concentration of Rat VEGF-A in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time: 3 h 30 min
Detection Range: 31.25-2000 pg/mL
Sensitivity: 18.75 pg/mL
Shipping Conditions: Ice bag
Storage Conditions: 2-8°C/-20°C, 12 months

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