Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit | Gentaur

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Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit

CAT:

763-E-EL-R0048-03

Size:

24 Tests

Price:

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For price, please contact [email protected].

Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit

Abbreviation: ACTH
Synonyms: Corticotropin;POMC;Adrenocorticotropin;
UniProt: PN/A1194
Species Reactivity: Rat
Type: Competitive-ELISA
Applications: ELISA
Field of Research: Cancer;Metabolism;Neuroscience;Signal transduction
Detection Method: Colormetric
Assay Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat ACTH. During the reaction, Rat ACTH in the sample or standard competes with a fixed amount of Rat ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time: 2 h 30 min
Detection Range: 15.63-1000 pg/mL
Sensitivity: 9.38 pg/mL
Shipping Conditions: Ice bag
Storage Conditions: 2-8°C/-20°C, 12 months

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