Mouse INS (Insulin) ELISA Kit

• Abbreviation:

  INS

• UniProt:

  P01325, P01326

• Cross Reactivity:

  This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed

• Target:

  IDDM2; ILPR; IRDN; MODY10

• Type:

  Sandwich-ELISA

• Applications:

  ELISA

• Field of Research:

  Cancer

• Assay Principle:

  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3 h 30 min

• Sample Type:

  Cell culture supernatant and tissue homogenate

• Detection Range:

  0.31-20 ng/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  0.19 ng/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Citation 01:

  https://www.nature.com/articles/s41419-021-04160-2

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S0963996922012650

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S1043661818301683

• Citation 04:

  https://pubs.acs.org/doi/abs/10.1021/acs.jafc.0c01467

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S0014482719303076

• Citation 06:

  https://link.springer.com/article/10.1007/s11356-018-1337-3

• Citation 07:

  https://www.spandidos-publications.com/10.3892/mmr.2022.12686

• Citation 08:

  https://www.hindawi.com/journals/ecam/2021/5514175/

• Citation 09:

  https://journals.sagepub.com/doi/abs/10.1177/1535370221997780

• Citation 10:

  https://link.springer.com/article/10.1007/s00795-019-00216-7

• Citation 11:

  https://link.springer.com/article/10.1007/s40200-022-01135-8

• Species:

  Mouse

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in cell culture supernatant and tissue homogenate.