Mouse SP-D (Pulmonary surfactant-associated protein D) ELISA Kit

CAT:

763-E-EL-M0993-01

Size:

96 Tests

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Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

Mouse SP-D (Pulmonary surfactant-associated protein D) ELISA Kit

Abbreviation: SP-D
CAS Number: 7732-18-5
UniProt: P5N/A4N/A4
Species Reactivity: Mouse
Type: Sandwich-ELISA
Applications: ELISA
Field of Research: Cancer;Cell Biology;Metabolism;Immunology;Microbiology;Signal transduction
Detection Method: Colormetric
Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SP-D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SP-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SP-D. You can calculate the concentration of Mouse SP-D in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time: 3 h 30 min
Detection Range: 0.16-10 ng/mL
Sensitivity: 0.1 ng/mL
Shipping Conditions: Ice bag
Storage Conditions: 2-8°C/-20°C, 12 months

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