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Mouse D2D (D-Dimer) ELISA Kit

CAT: 0763-E-EL-M0400-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-M0400-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
D2D
Cross Reactivity
This kit recognizes Mouse D2D in samples.No significant cross-reactivity or interference between Mouse D2D and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Cardiovascular
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse D2D. During the reaction, Mouse D2D in the sample or standard competes with a fixed amount of Mouse D2D on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse D2D. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse D2D in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Plasma
Detection Range
78.13-5000 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
46.88 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.nature.com/articles/s41589-022-01095-3
Citation 02
https://www.pnas.org/content/117/51/32648.short
Citation 03
https://www.sciencedirect.com/science/article/pii/S014296121630120X
Citation 04
https://www.sciencedirect.com/science/article/pii/S2473952922003111
Citation 05
https://www.sciencedirect.com/science/article/pii/S0006295219302886
Citation 06
https://www.frontiersin.org/articles/10.3389/fimmu.2019.01073/full
Citation 07
https://www.sciencedirect.com/science/article/pii/S0006291X23001031
Citation 08
https://www.sciencedirect.com/science/article/pii/S0895398819300455
Citation 09
https://link.springer.com/article/10.1186/s40635-021-00428-1
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse D2D concentrations in plasma.

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