Mouse C3 (Complement Component 3) ELISA Kit

CAT: 0763-E-EL-M0330-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0763-E-EL-M0330-01Size:24 Tests
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Abbreviation
C3
UniProt
P01027
Cross Reactivity
This kit recognizes Mouse C3 in samples. No significant cross-reactivity or interference between Mouse C3 and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Immunology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C3. You can calculate the concentration of Mouse C3 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
3.13-200 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
1.88 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.mdpi.com/2072-6651/12/12/794
Citation 02
https://www.mdpi.com/2115192
Citation 03
https://www.sciencedirect.com/science/article/pii/S0147651318301477
Citation 04
https://www.sciencedirect.com/science/article/pii/S0024320516306312
Citation 05
https://journals.sagepub.com/doi/abs/10.1177/0961203319898532
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse C3 concentrations in serum, plasma and other biological fluids.

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