Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit

Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit

• Abbreviation: EGFR2
• Synonyms: ERBB2; HER2; CD340; p185; MLN 19; NEU;
• CAS Number: 7732-18-5
• UniProt: P04626
• Species Reactivity: Human
• Cross Reactivity: This kit recognizes Human EGFR2 in samples.No significant cross-reactivity or interference between Human EGFR2 and analogues was observed
• Target: Human
• Type: Sandwich-ELISA
• Applications: ELISA
• Field of Research: Cancer; Immunology; Signal transduction; Tags and Cell Markers
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGFR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EGFR2. You can calculate the concentration of Human EGFR2 in the samples by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 3 h 30 min
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 31.25-2000 pg/mL
• Precision: Both intra-CV and inter-CV are < 10%
• Recovery: 80%-120%
• Sensitivity: 18.75 pg/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C/-20°C,12 months
• Applications Notes: This ELISA kit applies to the in vitro quantitative determination of Human EGFR2 concentrations in serum, plasma and other biological fluids.