Human VEGF-B (Vascular Endothelial Cell Growth Factor B) ELISA Kit

CAT:

763-E-EL-H2164-02

Size:

48 Tests

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Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

Human VEGF-B (Vascular Endothelial Cell Growth Factor B) ELISA Kit

Abbreviation: VEGF-B
Synonyms: VEGFB;VEGFL;VRF;
CAS Number: 7732-18-5
UniProt: P49765
Species Reactivity: Human
Type: Sandwich-ELISA
Applications: ELISA
Field of Research: Cancer;Cardiovascular;Neuroscience;Signal transduction
Detection Method: Colormetric
Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-B. You can calculate the concentration of Human VEGF-B in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time: 3 h 30 min
Detection Range: 15.63-1000 pg/mL
Sensitivity: 9.38 pg/mL
Shipping Conditions: Ice bag
Storage Conditions: 2-8°C/-20°C, 12 months

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