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Human CA19-9 (Carbohydrate antigen19-9) ELISA Kit

CAT: 0763-E-EL-H0637-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H0637-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
CA19-9
Cross Reactivity
This kit recognizes Human CA19-9 in samples. No significant cross-reactivity or interference between Human CA19-9 and analogues was observed
Target
Sialylated Lewis (a) Antigen; Cancer Antigen 19-9
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA19-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA19-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CA19-9. You can calculate the concentration of Human CA19-9 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
6.25-400 IU/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
3.75 IU/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.27687
Citation 02
https://pubs.acs.org/doi/abs/10.1021/acsabm.9b00616
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human CA19-9 concentrations in serum, plasma and other biological fluids.

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