FT4 (Free Thyroxine) ELISA Kit

• Abbreviation:

  FT4

• Cross Reactivity:

  This kit recognizes Universal fT4 in samples.No significant cross-reactivity or interference between Universal fT4 and analogues was observed

• Type:

  Competitive-ELISA

• Applications:

  ELISA

• Field of Research:

  Signaling transduction; Developmental Biology

• Assay Principle:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal fT4. During the reaction, Universal fT4 in the sample or standard competes with a fixed amount of Universal fT4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal fT4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal fT4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  1.56-100 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  0.94 pg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0269749121009040

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S004896972100262X

• Citation 03:

  https://www.mdpi.com/1422-0067/23/15/8244

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S2001037020304761

• Citation 05:

  https://www.hindawi.com/journals/omcl/2021/2482453/

• Citation 06:

  https://www.sciencedirect.com/science/article/pii/S0304389418300050

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S0147651319301538

• Citation 08:

  https://www.sciencedirect.com/science/article/pii/S0269749119349905

• Citation 09:

  https://jme.bioscientifica.com/view/journals/jme/67/3/JME-21-0095.xml

• Citation 10:

  https://www.nature.com/articles/s41598-021-00903-5

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/S0166432822003503

• Citation 12:

  https://www.sciencedirect.com/science/article/pii/S0045653515007572

• Detection Instrument:

  Microplate reader

• Species:

  Universal

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of fT4 concentrations in serum, plasma and other biological fluids.