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FT4 (Free Thyroxine) ELISA Kit

CAT: 0763-E-EL-0122-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-0122-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
FT4
Cross Reactivity
This kit recognizes Universal fT4 in samples.No significant cross-reactivity or interference between Universal fT4 and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Signaling transduction; Developmental Biology
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal fT4. During the reaction, Universal fT4 in the sample or standard competes with a fixed amount of Universal fT4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal fT4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal fT4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.94 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0269749121009040
Citation 02
https://www.sciencedirect.com/science/article/pii/S004896972100262X
Citation 03
https://www.mdpi.com/1422-0067/23/15/8244
Citation 04
https://www.sciencedirect.com/science/article/pii/S2001037020304761
Citation 05
https://www.hindawi.com/journals/omcl/2021/2482453/
Citation 06
https://www.sciencedirect.com/science/article/pii/S0304389418300050
Citation 07
https://www.sciencedirect.com/science/article/pii/S0147651319301538
Citation 08
https://www.sciencedirect.com/science/article/pii/S0269749119349905
Citation 09
https://jme.bioscientifica.com/view/journals/jme/67/3/JME-21-0095.xml
Citation 10
https://www.nature.com/articles/s41598-021-00903-5
Citation 11
https://www.sciencedirect.com/science/article/pii/S0166432822003503
Citation 12
https://www.sciencedirect.com/science/article/pii/S0045653515007572
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of fT4 concentrations in serum, plasma and other biological fluids.

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