LTE4 (Leukotriene E4) ELISA Kit

LTE4 (Leukotriene E4) ELISA Kit

• Abbreviation: LTE4
• Synonyms: LTE4
• CAS Number: 7732-18-5
• Species Reactivity: Universal
• Type: Competitive-ELISA
• Applications: ELISA
• Field of Research: Immunology
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LTE4. During the reaction, Universal LTE4 in the sample or standard competes with a fixed amount of Universal LTE4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LTE4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LTE4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 2 h 30 min
• Detection Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C/-20°C, 12 months