15d-PGJ2 (15-Deoxy-Delta12, 14-prostaglandin J2) ELISA Kit

15d-PGJ2 (15-Deoxy-Delta12, 14-prostaglandin J2) ELISA Kit

• Abbreviation: 15d-PGJ2
• Synonyms: 15-d-Δ12;14-PGJ2;
• CAS Number: 7732-18-5
• Species Reactivity: Universal
• Type: Competitive-ELISA
• Applications: ELISA
• Field of Research: Signal transduction
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 15d-PGJ2. During the reaction, Universal 15d-PGJ2 in the sample or standard competes with a fixed amount of Universal 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 15d-PGJ2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 15d-PGJ2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 2 h 30 min
• Detection Range: 3.13-200 ng/mL
• Sensitivity: 1.88 ng/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C/-20°C, 12 months