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SP (Substance P) ELISA Kit

CAT: 0763-E-EL-0067-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-0067-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
SP
UniProt
H:P20366; M:P41539; R:P06767
Cross Reactivity
This kit recognizes Universal SP in samples.No significant cross-reactivity or interference between Universal SP and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Neuroscience; Cardiovascular
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal SP. During the reaction, Universal SP in the sample or standard competes with a fixed amount of Universal SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal SP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal SP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
46.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://link.springer.com/article/10.1007/s00395-022-00968-x
Citation 02
https://www.mdpi.com/2072-6643/14/19/4083
Citation 03
https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03896f
Citation 04
https://www.sciencedirect.com/science/article/pii/S0944711321004153
Citation 05
https://www.sciencedirect.com/science/article/pii/S0378874121008746
Citation 06
https://www.sciencedirect.com/science/article/pii/S0378874120332967
Citation 07
https://www.sciencedirect.com/science/article/pii/S0378874121001513
Citation 08
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185139/
Citation 09
https://www.frontiersin.org/articles/10.3389/fmed.2020.00179/full
Citation 10
https://www.sciencedirect.com/science/article/pii/S0006899319304202
Citation 11
https://www.sciencedirect.com/science/article/pii/S0304394020307412
Citation 12
https://www.hindawi.com/journals/ecam/2020/1471824/
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of SP concentrations in serum, plasma and other biological fluids.

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