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MDA (Malondialdehyde) ELISA Kit

CAT: 0763-E-EL-0060-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-0060-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
MDA
Cross Reactivity
This kit recognizes Universal MDA in samples.No significant cross-reactivity or interference between Universal MDA and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Neuroscience; Signal transduction; Metabolism
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal MDA. During the reaction, Universal MDA in the sample or standard competes with a fixed amount of Universal MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal MDA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal MDA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S014296122200360X
Citation 02
https://link.springer.com/article/10.1007/s42765-022-00252-6
Citation 03
https://www.sciencedirect.com/science/article/pii/S2090123222001229
Citation 04
https://www.sciencedirect.com/science/article/pii/S2213231719315496
Citation 05
https://www.sciencedirect.com/science/article/pii/S0891584922009455
Citation 06
https://www.sciencedirect.com/science/article/pii/S0753332222010629
Citation 07
https://www.sciencedirect.com/science/article/pii/S0891584922001514
Citation 08
https://www.hindawi.com/journals/omcl/2022/9041914/
Citation 09
https://www.hindawi.com/journals/omcl/2022/7288729/
Citation 10
https://www.mdpi.com/1968532
Citation 11
https://www.sciencedirect.com/science/article/pii/S0147651322000240
Citation 12
https://www.mdpi.com/1188916
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of MDA concentrations in serum, plasma and other biological fluids.

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