PGE2 (Prostaglandin E2) ELISA Kit

• Abbreviation:

  PGE2

• Cross Reactivity:

  This kit recognizes Universal PGE2 in samples.No significant cross-reactivity or interference between Universal PGE2 and analogues was observed

• Target:

  PG-E2; Dinoprostone

• Type:

  Competitive-ELISA

• Applications:

  ELISA

• Field of Research:

  Signal Transduction

• Assay Principle:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal PGE2. During the reaction, Universal PGE2 in the sample or standard competes with a fixed amount of Universal PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGE2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal PGE2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  31.25-2000 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  18.75 pg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S1550413122004612

• Citation 02:

  https://immunology.sciencemag.org/content/4/36/eaau6426.abstract

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S0144861722009377

• Citation 04:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254128/

• Citation 05:

  https://academic.oup.com/ecco-jcc/article-abstract/9/9/708/346040

• Citation 06:

  https://elifesciences.org/articles/54523

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S0753332222010204

• Citation 08:

  https://www.sciencedirect.com/science/article/pii/S0891584921000058

• Citation 09:

  https://www.frontiersin.org/articles/10.3389/fimmu.2021.641874/full

• Citation 10:

  https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb01389d

• Citation 11:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352634/

• Citation 12:

  https://www.sciencedirect.com/science/article/pii/S0944711322004391

• Detection Instrument:

  Microplate reader

• Species:

  Universal

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of PGE2 concentrations in serum, plasma and other biological fluids.