ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit

ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit

• Abbreviation: ST;5-HT
• Synonyms: 5HT;Enteramine;Thrombocytin;Thrombotonin;3- (β-Aminoethyl) -5-hydroxyindole;
• CAS Number: 7732-18-5
• Species Reactivity: Universal
• Type: Competitive-ELISA
• Applications: ELISA
• Field of Research: Neuroscience
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ST/5-HT. During the reaction, Universal ST/5-HT in the sample or standard competes with a fixed amount of Universal ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ST/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ST/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 2 h 30 min
• Detection Range: 15.63-1000 ng/mL
• Sensitivity: 9.38 ng/mL
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C/-20°C, 12 months