8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

• Abbreviation:

  8-OHdG

• Cross Reactivity:

  This kit recognizes Universal 8-OHdG in samples.No significant cross-reactivity or interference between Universal 8-OHdG and analogues was observed

• Type:

  Competitive-ELISA

• Applications:

  ELISA

• Field of Research:

  Metabolism; Cardiovascular; Immunology; Cancer

• Assay Principle:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 8-OHdG. During the reaction, Universal 8-OHdG in the sample or standard competes with a fixed amount of Universal 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 8-OHdG in tested samples can be calculated by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  1.56-100 ng/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  0.94 ng/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Citation 01:

  https://www.nature.com/articles/s41586-020-2394-6

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S2213231721000203

• Citation 03:

  https://www.nature.com/articles/s41419-022-05120-0

• Citation 04:

  https://www.liebertpub.com/doi/abs/10.1089/ars.2020.8159

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S0147651322008405

• Citation 06:

  https://www.liebertpub.com/doi/abs/10.1089/ars.2022.0008

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S0891584922002295

• Citation 08:

  https://www.mdpi.com/1856270

• Citation 09:

  https://www.mdpi.com/article/10.3390/antiox11112264

• Citation 10:

  https://www.mdpi.com/2076-3921/11/7/1301

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/S1043661822000688

• Citation 12:

  https://www.frontiersin.org/articles/10.3389/fimmu.2022.880578/full

• Detection Instrument:

  Microplate reader

• Species:

  Universal

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of 8-OHdG concentrations in serum, plasma and other biological fluids.