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PGI2 (Prostacyclin) ELISA Kit

CAT: 0763-E-EL-0022-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-0022-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
PGI2
Cross Reactivity
This kit recognizes Universal PGI2 in samples.No significant cross-reactivity or interference between Universal PGI2 and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Cardiovascular
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal PGI2. During the reaction, Universal PGI2 in the sample or standard competes with a fixed amount of Universal PGI2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGI2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal PGI2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://cmjournal.biomedcentral.com/articles/10.1186/s13020-021-00424-3
Citation 02
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868340/
Citation 03
https://www.nature.com/articles/s41598-021-90605-9
Citation 04
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7547127/
Citation 05
https://www.hindawi.com/journals/cmmi/2022/6177809/
Citation 06
https://jpet.aspetjournals.org/content/375/1/40.abstract
Citation 07
https://journals.sagepub.com/doi/abs/10.1177/0885328218774512
Citation 08
https://www.tandfonline.com/doi/abs/10.3402/fnr.v59.29046
Citation 09
https://www.tandfonline.com/doi/abs/10.1080/13813455.2020.1780263
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of PGI2 concentrations in serum, plasma and other biological fluids.

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