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RecombinantMaspin, Human

CAT: 0013-GTR18990489-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18990489-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Maspin is a non-inhibitory member of the serpin superfamily, a group of 40-60 kDa serine protease inhibitors. Maspin was discovered in 1994 and has been identified in prostate, thymus, testis, intestine, tongue, and lung. Recently Maspin received a great deal of attention due to its potential apoptotic role in cancers. Maspin expression predicts a better prognosis for several types of carcinomas including breast, prostate, colon, and oral squamus cell carcinoma. The level of Maspin is also inversely correlated to VEGF-A, an angiogenic factor. Researchers found that Maspin may play its apoptotic role as an endogenous inhibitor of histone deacetylase I (HDAC I) . Therefore, Maspin is a potential personalized marker of Non-Small Cell Lung Carcinoma (NSCLC) .Recombinant human Maspin (rhMaspin) produced in E.coli is a single non-glycosylated polypeptide chain containing 375 amino acids. rhMaspin has a molecular mass of 42.1 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Product Name Alternative
Serpin B5, PI-5
Source
Escherichia coli.
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analysis.
Bioactivity
Data not available.
Form
Lyophilized after extensive dialysis against PBS.
Molecular Weight
42.1 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant human Maspin (rhMaspin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMaspin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/mL.
Preservative
Lyophilized after extensive dialysis against PBS.
AA Sequence
MDALQLANS AFAVDLFKQL CEKEPLGNVL FSPICLSTSL SLAQVGAKGD TANEIGQVLH FENVKDIPFG FQTVTSDVNK LSSFYSLKLI KRLYVDKSLN LSTEFISSTK RPYAKELETV DFKDKLEETK GQINNSIKDL TDGHFENILA DNSVNDQTKI LVVNAAYFVG KWMKKFPESE TKECPFRLNK TDTKPVQMMN MEATFCMGNI DSINCKIIEL PFQNKHLSMF ILLPKDVEDE STGLEKIEKQ LNSESLSQWT NPSTMANAKV KLSIPKFKVE KMIDPKACLE NLGLKHIFSE DTSDFSGMSE TKGVALSNVI HKVCLEITED GGDSIEVPGA RILQHKDELN ADHPFIYIIR HNKTRNIIFF GKFCSP

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