RecombinantSDF-1α/CXCL12, Mouse

SDF-1 α and SDF-1 β, members of the chemokine α subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. SDF-1 α and SDF-1 β cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1 α and SDF-1 β are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 β and absent from SDF-1 α. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF acts via the chemokine receptor CXCR4 and has been shown to be a chemoattractant for T-lymphocytes, monocytes, pro- and pre-B cells, but not neutrophils. Mice lacking SDF-1 or CXCR4 have been found to have impaired B-lymphopoiesis, myelopoiesis, vascular development, cardiogenesis and abnormal neuronal cell migration and patterning in the central nervous system.Recombinant Mouse SDF-1 α/CXCL12 produced in CHO cells is a polypeptide chain containing 68 amino acids. A fully biologically active molecule, rm SDF-1 β/CXCL12 has a molecular mass of 8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

SDF-1 β, Stromal-Cell Derived Factor-1, CXCL12, PBSF

CHO

Immunology & Inflammation

< 0.2 EU/μg, determined by LAL method.

> 95% as analyzed by SDS-PAGE.

The EC50 value of mouse SDF-1 α/CXCL12 on Caˆ2+ mobilization assay in CHO-K1/Gα15/mCXCR4 cells (human Gα15 and mCXCR4 stably expressed in CHO-K1 cells) is less than 1.5 μg/ml.

Lyophilized after extensive dialysis against PBS.

8 kDa, observed by reducing SDS-PAGE.

Lyophilized recombinant Mouse SDF‐1 α/CXCL12 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse SDF‐1 α/CXCL12 should be stable up to 1 week at 4°C or up to 3 months at -20°C.

For research use only.

Reconstituted in ddH2O or PBS at 100 μg/ml.

Lyophilized after extensive dialysis against PBS.