EGK_07847 Protein

CAT: 0013-GTR18989715-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18989715-01Size:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

This EGK_07847 Protein spans the amino acid sequence from region 24-734aa. Purity: Greater than 95% as determined by SDS-PAGE.

Product Name Alternative

(EGK_07847)

UniProt

H9FNS0

Expression Region

24-734aa

Tag

C-terminal 10xHis-tagged

Source

Mammalian cell

Origin

Macaca mulatta (Rhesus macaque)

Field of Research

Immunology & Inflammation

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 1.943-2.724 ng/mL.

Form

Lyophilized powder

Molecular Weight

81.9 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Preservative

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

AA Sequence

FAPIPRITWEHREVRLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSSLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSIAEEVFSHGKYMQSATVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGRRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALYQTESPSRGLVQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPVPTSTSCEPKIVINTVPQLHSEKTMYLKSSDNR

UniProtKB · G7NEM8

Semaphorin-4D

G7NEM8_MACMU · Macaca mulatta

View on UniProt ↗

Primary accession: G7NEM8
Review status: UniProtKB unreviewed (TrEMBL)
Gene: SEMA4D
Protein existence: 2: Evidence at transcript level
Organism: Macaca mulatta (Rhesus macaque)
Taxonomy ID: 9544
Alternative names: —
EC number: —
Processing: —
Secondary accessions: H9FNS0

Protein keywords

Cellular component

Cell membraneMembraneSecreted

Molecular function

Developmental protein

Biological process

DifferentiationNeurogenesis

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

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