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Human BDNF Protein

CAT: 0013-GTR18986379-01Size: 2 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18986379-01Size:2 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant of human BDNF protein
Product Name Alternative
Brain-Derived Neurotrophic Factor, BDNF, MGC34632.
Source
Escherichia Coli
Field of Research
Neuroscience
Purity
BDNF is greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bioactivity
The ED50, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2μg/ml.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions
Stability: Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Protein content: BDNF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) . 2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard
Preservative
The protein was lyophilized without any additives.
AA Sequence
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

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