Products for Research Use Only

Human Leptin Protein

CAT: 0013-GTR18984908-01Size: 200 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18984908-01Size:200 µg
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Description
Recombinant of human Leptin protein
Product Name Alternative
OB Protein, Obesity Protein, OBS, Obesity factor.
Source
Escherichia Coli
Field of Research
Metabolism Research
Purity
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Bioactivity
Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.
Storage Conditions
Stability: Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C
Notes
For research use only.
Applications Notes
Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.878 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) . 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard
Preservative
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
AA Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln

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