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MMLV RT Protein

CAT: 0013-GTR18983553-01Size: 2500 UDry Ice: NoHazardous: No
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CAT#:0013-GTR18983553-01Size:2500 U
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Moloney Murine Leukemia Virus Reverse Trancscriptase protein
Source
Recombinant E. coli strain
Form
Sterile Filtered clear solution (200 U/μl) .
Storage Conditions
Stability: Stable for 5 days at 10°C, for longer period of time store at -20°C. Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly (A) -oligo (dT) 12-18 as the template-primer. Standard cDNA Synthesis Conditions50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42oC
Preservative
50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol.

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