Products for Research Use Only
RegisterLogin

Protease Protein

CAT: 0013-GTR18983391-01Size: 500 IUDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18983391-01Size:500 IU
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Recombinant of Protease protein
Source
Escherichia Coli
Field of Research
Protein Biochemistry
Form
Sterile filtered colorless solution.
Storage Conditions
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles
Notes
For research use only.
Applications Notes
Unit Definition: One unit will cleave ?90% of 100 μg of a test GST-fusion protein in Cleavage Buffer (50mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 25°C) at 5°C for 16 hours. Cleavage Buffer: 50mM Tris-HCl, pH-7.0 (at 25°C), 150mM NaCl, 1mM EDTA, 1mM dithiothreitol. Chill to 5°C prior to use. Cleavage Conditions: For Cleavage of a Fusion Protein: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease

Related Products

CatalogName
GTR19092035-01Recombinant Proteus mirabilis Protease HtpX (htpX)
GTR18712506-01HIV Protease, Virus Protease Antibody
GTR18910477Chymotrypsin-Like Protease Recombinant Protein
GTR18987581-01Human Cysteine protease ATG4A Protein
GTR19155203-01Protease K
GTR19253889-01FabCOUPER protease
GTR19266709-01IdeS protease
GTR19122163-013C Protease
GTR19122158-01TEV Protease
GTR18006831Protease Antibody

Alternative Products

CatalogName
abx073528-01Protease Protein
abx620811-01Cysteine Protease Protein
32-2735-150Protease Recombinant Protein
30-031001HIV1 protease IIIB protein
abx263019-01HIV-1 Protease Protein
PO49351E1TEV protease Recombinant Protein
abx263020-01HIV-2 Protease Protein
376558LONP1 (hLON, hLON ATP Dependent Protease, LON, LONP1, Ion Peptidase 1, Mitochondrial, LON Protease, Lon Protease Homolog, Lon Protease Like Protein, Lon Protease-like Protein) discontinued
MBS6007754-01LONP1 (PRSS15, Lon Protease Homolog, Mitochondrial, LONHs, Lon Protease-like Protein, Mitochondrial ATP-dependent Protease Lon, Serine Protease 15)
AK1391-1000BSA Protease Free (Protein Stabilizing)

Popular Products