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MutS Protein

CAT: 0013-GTR18981004-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18981004-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant MutS protein
Product Name Alternative
MutS, Thermus Aquaticus DNA Mismatch Repair Protein, DNA mismatch repair protein MutS.
Source
Escherichia Coli
Purity
Greater than 95.0% as determined by SDS-PAGE.
Form
Sterile filtered colorless solution.
Storage Conditions
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid multiple freeze-thaw cycles
Notes
For research use only.
Applications Notes
Applications: Recommended Applications: 1. Remove mismatch DNA (error correction) from gene synthesis reaction 2. Mutation detection and removal 3. Rapid isothermal SNP detection. Preparation protocol: 1. After first round PCR, purify PCR fragments using Qiagen QIAquick PCR purification kit with elution in dH2O. 2. Dilute PCR product to 250 ng/μl in 10 mM Tris–HCl, pH 7.8, 50 mM NaCl and heat to 95 °C for 5 min followed by cooling at 0.1°C /s to 25 °C. 3. Add binding buffer (20 mM Tris–HCl, pH 7.8, 10 mM NaCl, 5 mM MgCl2, 1 mM DTT and 5% glycerol) to annealed PCR product, adjust DNA concentration to 11.5 ng/μl, add MutS dimers to 950 nM. 4. Incubate the mixture at room temperature for 10 min, then add an equal volume of Ni-NTA beads pre-equilibrated with 1xbinding buffer, and incubate for 30 min at room temperature. 5. Gently spin down beads and save supernatant for subsequent processing (second round PCR, cloning etc) . Reaction Conditions: 100mM KCl, 50mM Tris-HCl, pH-8.5, 20mM MgCl2, 0.1mM EDTA, 1mM DTT and 2% Glycerol, 65 °C
Preservative
The MutS protein is supplied in 20mM Tris-HCl, pH-8, 250mM NaCl, 0.1mM EDTA, 1mM DTT and 50% Glycerol.
AA Sequence
MEGMLKGEGPGPLPPLLQQYVELRDQYPDYLLLFQVGDFYECFGEDAERLARALG LVLTHKTSKDFTTPMAGIPLRAFEAYAERLLKMGFRLAVADQVEPAEEAEGLVRREV TQLLTPGTLLQESLLPREANYLAAIATGDGWGLAFLDVSTGEFKGTVLKSKSALYDELF RHRPAEVLLAPELLENGAFLDEFRKRFPVMLSEAPFEPEGEGPLALRRARGALLAYAQ RTQGGALSLQPFRFYDPGAFMRLPEATLRALEVFEPLRGQDTLFSVLDETRTAPGRRL LQSWLRHPLLDRGPLEARLDRVEGFVREGALREGVRRLLYRLADLERLATRLELGRASP KDLGALRRSLQILPELRALLGEEVGLPDLSPLKEELEAALVEDPPLKVSEGGLIREGYDPD LDALRAAHREGVAYFLELEERERERTGIPTLKVGYNAVFGYYLEVTRPYYERVPKEYRPV QTLKDRQRYTLPEMKEKEREVYRLEALIRRREEEVFLEVRERAKRQAEALREAARILAEL DVYAALAEVAVRYGYVRPRFGDRLQIRAGRHPVVERRTEFVPNDLEMAHELVLITGPN MAGKSTFLRQTALIALLAQVGSFVPAEEAHLPLFDGIYTRIGASDDLAGGKSTFMVEM EEVALILKEATENSLVLLDEVGRGTSSLDGVAIATAVAEALHERRAYTLFATHYFELTAL GLPRLKNLHVAAREEAGGLVFYHQVLPGPASKSYGVEVAAMAGLPKEVVARARALLQ

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