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Sheep IgM Antibody

CAT: 0013-GTR18958221Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18958221Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Sheep IgM Antibody
Product Name Alternative
Sheep Immunoglobulin M
Conjugation
Unconjugated
Source
Sheep
Origin
Sheep
Field of Research
Protein Biochemistry
Concentration
1.0 mg/mL
Dilution
ELISA: User optimized, IHC: User Optimized, IP: User optimized, WB: User optimized
Purity
Sheep IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Sheep IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and anti-Sheep IgM (μ chain specific) . No reaction was observed against anti-Sheep IgG F (c) . Some light chain cross reactivity will occur with anti-Sheep IgG.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Sheep IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Sheep IgM whole molecule is stable at 4° C prior to restoration. It is recommended to aliquot restored Sheep IgM whole molecule after adding equal parts glycerol for storage at -20° C for extended storage and to prevent repeated freeze-thaw cycles.
Tested Applications
SDS-PAGE
Preservative
Preservative: 0.1% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

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