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USP16 Antibody

CAT: 0013-GTR17698008Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17698008Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to USP16
Product Name Alternative
Anti USP16 antibody, anti ubiquitin specific peptidase 16 antibody, anti UBP-M antibody, anti deubiquitinating enzyme 16 antibody, anti ubiquitin carboxyl-terminal hydrolase 16 antibody, anti ubiquitin specific protease 16 antibody, anti ubiquitin thiolesterase 16 antibody, anti ubiquitin-specific processing protease 16 antibody
Target
USP16
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
NVEWNICQDCKTDNK
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.5 μg/ml
Molecular Weight
93.6; 93.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 50kDa and 26kDa in lysates of cell lines HEK293, A549 and HeLa after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.5kDa according to NP_006438.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Equine, Human, Mouse, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
10600

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