Anti-Sperm-associated antigen 5 SPAG5 Antibody

• Description:

  Boster Bio Anti-Sperm-associated antigen 5 SPAG5 Antibody catalog # A07062. Tested in WB applications. This antibody reacts with Human.

• Synonyms:

  Sperm-associated antigen 5; Astrin; Deepest; Mitotic spindle-associated protein p126; MAP126; SPAG5;

• Gene Name:

  SPAG5

• UniProt:

  Q96R06

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide corresponding to residues in Human SPAG5

• Clonality:

  Polyclonal

• Tissue Specificity:

  Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.

• Applications:

  WB

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Dilution:

  WB: 1:500-1:1000

• Form:

  Liquid

• Function:

  Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074) . Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074) . In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792) . Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806) . In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116) . May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457) .

• Molecular Weight:

  134422 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Sperm-associated antigen 5

• Subcellular Location:

  Cytoplasm

• Protein Name:

  Sperm-associated antigen 5