Anti-Nephrocystin-1 NPHP1 Antibody

• Description:

  Boster Bio Anti-Nephrocystin-1 NPHP1 Antibody catalog # A03029. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Nephrocystin-1; Juvenile nephronophthisis 1 protein; NPHP1; NPH1;

• Gene Name:

  NPHP1

• UniProt:

  O15259

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to residues in Human Nephrocystin-1.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widespread expression, with highest levels in pituitary gland, spinal cord, thyroid gland, testis, skeletal muscle, lymph node and trachea. Weakly expressed in heart, kidney and pancreas. Expressed in nasal epithelial cells (at protein level) .

• Applications:

  WB

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Dilution:

  WB: 1:500-1:1000

• Form:

  Liquid

• Function:

  Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8 (By similarity) . Does not seem to be strictly required for ciliogenesis (By similarity) . Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling. May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development. In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis (By similarity) . .

• Molecular Weight:

  83299 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Nephrocystin-1

• Subcellular Location:

  Cell junction, adherens junction . Cell projection, cilium. Cytoplasm, cytoskeleton, cilium axoneme. Cell junction, tight junction. Colocalizes with E-cadherin and BCAR1 at or near the cell-cell adherens junctions (By similarity) . Localized to respiratory cilia axoneme. Localized to the transition zone of respiratory cilia, photoreceptor-connecting cilia and renal monocilia. In cultured renal cells, it localizes diffusely in the cytoplasm but, as cells approach confluence, it accumulates to basolateral tight junctions. .

• Protein Name:

  Nephrocystin-1