Anti-CD33 Antibody

• Description:

  Boster Bio Anti-CD33 Antibody catalog # A01508-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD33; CD33; SIGLEC3;

• Gene Name:

  CD33

• UniProt:

  P20138

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human SIGLEC3/CD33 (NP_001763.3) .

• Clonality:

  Polyclonal

• Tissue Specificity:

  Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells.

• Applications:

  WB, IHC, ICC, IF

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Form:

  Liquid

• Function:

  Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212) . Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid- bearing glycans (PubMed:7718872) . Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine- based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109) . These phosphorylations provide docking sites for the recruitment and activation of protein- tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109) . In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109) . One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323) .

• Molecular Weight:

  39825 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Myeloid cell surface antigen CD33

• Subcellular Location:

  Isoform CD33M: Cell membrane

• Protein Name:

  Myeloid cell surface antigen CD33