Anti-RUNX1 (phospho-S276) Antibody

• Description:

  Boster Bio Anti-RUNX1 (phospho-S276) Antibody catalog # A00086S276. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Runt-related transcription factor 1; Acute myeloid leukemia 1 protein; Core-binding factor subunit alpha-2; CBF-alpha-2; Oncogene AML-1; Polyomavirus enhancer-binding protein 2 alpha B subunit; PEA2-alpha B; PEBP2-alpha B; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; RUNX1; AML1, CBFA2;

• Gene Name:

  RUNX1

• UniProt:

  Q01196

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human RUNX1 around the phosphorylation site of Serine 276.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

• Applications:

  WB

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Dilution:

  WB: 1:500-1:1000

• Form:

  Liquid

• Function:

  Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA- binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable) . Essential for the development of normal hematopoiesis (PubMed:17431401) . Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218) . Inhibits KAT6B-dependent transcriptional activation (By similarity) . Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity) . Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532) . Positively regulates the expression of RORC in T-helper 17 cells (By similarity) .

• Molecular Weight:

  48737 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Runt-related transcription factor 1

• Subcellular Location:

  Nucleus.

• Protein Name:

  Runt-related transcription factor 1