Anti-TCP1 theta/CCT8 Antibody Picoband®, Biotine Conjugate

Anti-TCP1 theta/CCT8 Antibody Picoband®, Biotine Conjugate

• Background: T-complex protein 1 subunit theta is a protein that in humans is encoded by the CCT8 gene. This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1.
• Description: Boster Bio Anti-TCP1 theta/CCT8 Antibody Picoband® catalog # PB9993. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: T-complex protein 1 subunit theta;TCP-1-theta;CCT-theta;Renal carcinoma antigen NY-REN-15;CCT8;C21orf112, CCTQ, KIAA0002;
• Gene Name: T-complex protein 1 subunit theta
• Gene ID: 10694
• UniProt: P50990
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human CCT8 recombinant protein (Position: K421-D548). Human CCT8 shares 98.4% amino acid (aa) sequence identity with mouse CCT8.
• Clonality: Polyclonal
• Applications: Flow Cytometry,IF,IHC,IHC-F,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. .
• References & Citations: 1. "Entrez Gene: CCT8 chaperonin containing TCP1, subunit 8 (theta)". 2. Kubota H, Hynes G, Willison K (Apr 1995). "The eighth Cct gene, Cctq, encoding the theta subunit of the cytosolic chaperonin containing TCP-1". Gene 154 (2): 231–6.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 59621 MW
• Observed Molecular Weight: 60 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
  Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human