Anti-BMP2 Antibody Picoband®-FITC Conjugate

Anti-BMP2 Antibody Picoband®-FITC Conjugate

• Background: BMP2 is also known as Bone morphogenetic protein 2 or BMP2A. It is mapped to 20p12. The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily.?BMP-2, like other?bone morphogenetic proteins,?plays an important role in the development of bone and cartilage. It is involved in the?hedgehog pathway,?TGF beta signaling pathway, and in?cytokine-cytokine receptor interaction. Also, it is involved in cardiac cell differentiation and?epithelial to mesenchymal transition. In addition, BMP2A has been suggested as a reasonable candidate for the human condition fibrodysplasia (myositis) ossificans progressiva, on the basis of observations in a Drosophila model.
• Description: Boster Bio Anti-BMP2 Antibody Picoband® catalog # PB9687. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;BMP2;BMP2A;
• Gene Name: Bone morphogenetic protein 2
• Gene ID: 650
• UniProt: P12643
• Host: Rabbit
• Reactivity: Human,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human BMP-2, identical to the related mouse and rat sequences.
• Clonality: Polyclonal
• Applications: ELISA,IHC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451). .
• References & Citations: 1. Gopal Rao, V. V. N., Loffler, C., Wozney, J. M., Hansmann, I. The gene for bone morphogenetic protein 2A (BMP2A) is localized to human chromosome 20p12 by radioactive and nonradioactive in situ hybridization. Hum. Genet. 90: 299-302, 1992.  2. Kaplan, F. S., Tabas, J. A., Zasloff, M. A. Fibrodysplasia ossificans progressiva: a clue from the fly? Calcif. Tissue Int. 47: 117-125, 1990.  3. Sampath TK, Coughlin JE, Whetstone RM, Banach D, Corbett C, Ridge RJ, Ozkaynak E, Oppermann H, Rueger DC (August 1990)."Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily". J. Biol. Chem. 265 (22): 13198–205.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 44702 MW
• Observed Molecular Weight: 20 kDa, 40 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
  ELISA(Cap) , 1-5μg/ml, Human, -